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1x phusion reaction buffer  (New England Biolabs)


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    Structured Review

    New England Biolabs 1x phusion reaction buffer
    1x Phusion Reaction Buffer, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 96/100, based on 1637 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/1x+phusion+reaction+buffer/Phusion+HF+Buffer/pm28829124__cb7b00636_si_001-14-10-14
    Average 96 stars, based on 1637 article reviews
    1x phusion reaction buffer - by Bioz Stars, 2026-09
    96/100 stars

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    Related Articles

    Cloning:

    Article Title: 8-Oxo-7,8-dihydroguanine in the Context of a Gene Promoter G-Quadruplex Is an On–Off Switch for Transcription
    Article Snippet: .. The cloning experiment was conducted in a 25-μL reaction with 1x Phusion reaction buffer (NEB), 200 μM dNTPs, 1 μM each of the forward and reverse primers, 1,000 ng of psiCHECK2 plasmid, 3% DMSO, and 0.5 units of Phusion DNA polymerase. ..

    Article Title: Promoters vs. telomeres: AP-endonuclease 1 interactions with abasic sites in G-quadruplex folds depend on topology
    Article Snippet: .. The cloning experiment was conducted in a 25-μL reaction with 1x Phusion reaction buffer (NEB), 200 μM dNTPs, 1 μM each of the forward and reverse primers, 1,000 ng of psiCHECK2 plasmid, 3% DMSO, and 0.5 units of Phusion DNA polymerase. ..

    Article Title: Human DNA Repair Genes Possess Potential G-Quadruplex Sequences in Their Promoters and 5'-Untranslated Regions.
    Article Snippet: .. The cloning experiment was conducted in a 25-μL reaction with 1x Phusion reaction buffer (NEB), 200 μM dNTPs, 1 μM each of the forward and reverse primers, 1,000 ng of psiCHECK2 plasmid, 3% DMSO, and 0.5 units of Phusion DNA polymerase. ..

    Article Title: Human NEIL3 Gene Expression Regulated by Epigenetic-Like Oxidative DNA Modification
    Article Snippet: .. The cloning experiment was conducted in a 25-μL reaction with 1x Phusion reaction buffer (NEB), 200 μM dNTPs, 1 μM each of the forward and reverse primers, 1,000 ng of psiCHECK2 plasmid, 3% DMSO, and 0.5 units of Phusion DNA polymerase. ..

    Plasmid Preparation:

    Article Title: 8-Oxo-7,8-dihydroguanine in the Context of a Gene Promoter G-Quadruplex Is an On–Off Switch for Transcription
    Article Snippet: .. The cloning experiment was conducted in a 25-μL reaction with 1x Phusion reaction buffer (NEB), 200 μM dNTPs, 1 μM each of the forward and reverse primers, 1,000 ng of psiCHECK2 plasmid, 3% DMSO, and 0.5 units of Phusion DNA polymerase. ..

    Article Title: Promoters vs. telomeres: AP-endonuclease 1 interactions with abasic sites in G-quadruplex folds depend on topology
    Article Snippet: .. The cloning experiment was conducted in a 25-μL reaction with 1x Phusion reaction buffer (NEB), 200 μM dNTPs, 1 μM each of the forward and reverse primers, 1,000 ng of psiCHECK2 plasmid, 3% DMSO, and 0.5 units of Phusion DNA polymerase. ..

    Article Title: Human DNA Repair Genes Possess Potential G-Quadruplex Sequences in Their Promoters and 5'-Untranslated Regions.
    Article Snippet: .. The cloning experiment was conducted in a 25-μL reaction with 1x Phusion reaction buffer (NEB), 200 μM dNTPs, 1 μM each of the forward and reverse primers, 1,000 ng of psiCHECK2 plasmid, 3% DMSO, and 0.5 units of Phusion DNA polymerase. ..

    Article Title: Human NEIL3 Gene Expression Regulated by Epigenetic-Like Oxidative DNA Modification
    Article Snippet: .. The cloning experiment was conducted in a 25-μL reaction with 1x Phusion reaction buffer (NEB), 200 μM dNTPs, 1 μM each of the forward and reverse primers, 1,000 ng of psiCHECK2 plasmid, 3% DMSO, and 0.5 units of Phusion DNA polymerase. ..

    Polymerase Chain Reaction:

    Article Title: CPSF6 Promotes HIV-1 Preintegration Complex Function
    Article Snippet: .. Briefly, the first-round PCR was performed in a final volume of 50 μL containing 100 ng of total DNA from infected cells, 1X Phusion reaction buffer (NEB), deoxy-nucleotide triphosphate (dNTP) nucleotide mix containing 200 μM concentrations of each nucleotide (Promega), 500 nM (each) of primers that target the host chromosomal Alu repeat sequence (5’-GCCTCCCAAAGTGCTGGGATTACAG-3’) and HIV-1 Gag sequence (5’-GTTCCTGCTATGTCACTTCC-3’), and 1.25 U of Phusion DNA Polymerase (NEB). ..

    Infection:

    Article Title: CPSF6 Promotes HIV-1 Preintegration Complex Function
    Article Snippet: .. Briefly, the first-round PCR was performed in a final volume of 50 μL containing 100 ng of total DNA from infected cells, 1X Phusion reaction buffer (NEB), deoxy-nucleotide triphosphate (dNTP) nucleotide mix containing 200 μM concentrations of each nucleotide (Promega), 500 nM (each) of primers that target the host chromosomal Alu repeat sequence (5’-GCCTCCCAAAGTGCTGGGATTACAG-3’) and HIV-1 Gag sequence (5’-GTTCCTGCTATGTCACTTCC-3’), and 1.25 U of Phusion DNA Polymerase (NEB). ..

    Sequencing:

    Article Title: CPSF6 Promotes HIV-1 Preintegration Complex Function
    Article Snippet: .. Briefly, the first-round PCR was performed in a final volume of 50 μL containing 100 ng of total DNA from infected cells, 1X Phusion reaction buffer (NEB), deoxy-nucleotide triphosphate (dNTP) nucleotide mix containing 200 μM concentrations of each nucleotide (Promega), 500 nM (each) of primers that target the host chromosomal Alu repeat sequence (5’-GCCTCCCAAAGTGCTGGGATTACAG-3’) and HIV-1 Gag sequence (5’-GTTCCTGCTATGTCACTTCC-3’), and 1.25 U of Phusion DNA Polymerase (NEB). ..



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